Coding

Part:BBa_K4212009:Design

Designed by: Shirin Bamezai   Group: iGEM22_Imperial_College_London   (2022-09-29)


GerAK_chimera


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 2384
    Illegal EcoRI site found at 4339
    Illegal PstI site found at 533
    Illegal PstI site found at 4290
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 2384
    Illegal EcoRI site found at 4339
    Illegal NheI site found at 4
    Illegal PstI site found at 533
    Illegal PstI site found at 4290
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 2384
    Illegal EcoRI site found at 4339
    Illegal BglII site found at 3871
    Illegal XhoI site found at 640
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 2384
    Illegal EcoRI site found at 4339
    Illegal PstI site found at 533
    Illegal PstI site found at 4290
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 2384
    Illegal EcoRI site found at 4339
    Illegal PstI site found at 533
    Illegal PstI site found at 4290
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 3526


Design Notes

Given that the GerAA, GerAC and GerKB came from different operons in the B. subtilis genome, the same had to be refactored. Careful consideration was made in regards to the native upstream region used in front of the GerKB coding sequence, which contains the RBS.


Source

Synthetic construct using native coding sequences from GerA sub-units and GerK sub-units of B. subtilis.

References